Transposon impala, a novel tool for gene tagging in the rice blast fungus Magnaporthe grisea.

@article{Villalba2001TransposonIA,
  title={Transposon impala, a novel tool for gene tagging in the rice blast fungus Magnaporthe grisea.},
  author={F. Garcia Villalba and Marc Henri Lebrun and Aur{\'e}lie Hua-Van and Marie Jos{\'e}e Daboussi and Marie-Claire Grosjean-Cournoyer},
  journal={Molecular plant-microbe interactions : MPMI},
  year={2001},
  volume={14 3},
  pages={308-15}
}
impala, a Tc1-mariner transposable element from Fusarium oxysporum, was introduced into the rice blast fungus Magnaporthe grisea to develop transposon-based insertional mutagenesis. A construct (pNIL160) containing an autonomous impala copy inserted in the promoter of niaD encoding Aspergillus nidulans nitrate reductase was introduced by transformation into a M. grisea nitrate reductase-deficient mutant. impala excision was monitored by restoration of prototrophy for nitrate. Southern analysis… CONTINUE READING

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