Transposon Tc1-derived, sequence-tagged sites in Caenorhabditis elegans as markers for gene mapping.

@article{Korswagen1996TransposonTS,
  title={Transposon Tc1-derived, sequence-tagged sites in Caenorhabditis elegans as markers for gene mapping.},
  author={Hendrik C. Korswagen and Richard Michael Durbin and M. T. Smits and Ronald H. A. Plasterk},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1996},
  volume={93 25},
  pages={14680-5}
}
We present an approach to map large numbers of Tc1 transposon insertions in the genome of Caenorhabditis elegans. Strains have been described that contain up to 500 polymorphic Tc1 insertions. From these we have cloned and shotgun sequenced over 2000 Tc1 flanks, resulting in an estimated set of 400 or more distinct Tc1 insertion alleles. Alignment of these sequences revealed a weak Tc1 insertion site consensus sequence that was symmetric around the invariant TA target site and reads CAYATATRTG… CONTINUE READING

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