Transposome mutagenesis of an integral membrane transporter in Corynebacterium matruchotii.

@article{Wang2006TransposomeMO,
  title={Transposome mutagenesis of an integral membrane transporter in Corynebacterium matruchotii.},
  author={Cindy X. Wang and Barry Hayes and Martha M. Vestling and Kuni Takayama},
  journal={Biochemical and biophysical research communications},
  year={2006},
  volume={340 3},
  pages={953-60}
}
A transposon-5 insertion library of Corynebacterium matruchotii ATCC14266 was generated and screened for mutants with altered corynomycolic acid content. One of these designated 319 mutants showed an interruption of a gene encoding an integral membrane protein. MALDI mass spectra of trehalose monocorynomycolate (TMCM), trehalose dicorynomycolate, and methyl corynomycolates derived from cell wall arabinogalactan-corynomycolate showed that these lipids from the mutant contained a lower amount of… CONTINUE READING