Transposition of cloned P elements into Drosophila germ line chromosomes.

@article{Spradling1982TranspositionOC,
  title={Transposition of cloned P elements into Drosophila germ line chromosomes.},
  author={Allan C Spradling and Gerald M. Rubin},
  journal={Science},
  year={1982},
  volume={218 4570},
  pages={341-7}
}
Recombinant DNA carrying the 3-kilobase transposable element was injected into Drosophila embryos of a strain that lacked such elements. Under optimum conditions, half of the surviving embryos showed evidence of P element-induced mutations in a fraction of their progeny. Direct analysis of the DNA of strains derived from such flies showed them to contain from one to five intact 3-kilobase P elements located at a wide variety of chromosomal sites. DNA sequences located outside the P element on… CONTINUE READING
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