Transposition of Tn7 in Pseudomonas aeruginosa and isolation of alk::Tn7 mutations.

@article{Fennewald1979TranspositionOT,
  title={Transposition of Tn7 in Pseudomonas aeruginosa and isolation of alk::Tn7 mutations.},
  author={Michael Fennewald and James A. Shapiro},
  journal={Journal of bacteriology},
  year={1979},
  volume={139 1},
  pages={264-9}
}
Conjugal crosses with Pseudomonas aeruginosa donors carrying the CAM-OCT and RP4::Tn7 plasmids result in transfer of the Tn7 trimethoprim resistance (Tp(r)) determinant independently of RP4 markers. All Tp(r) exconjugants which lack RP4 markers have CAM-OCT genes and therefore must have received CAM-OCT::Tn7 plasmids formed by transposition of Tn7 from RP4::Tn7 to CAM-OCT. Most crosses yield exconjugants carrying mutant CAM-OCT plasmids which no longer determine either camphor or alkane… CONTINUE READING