Transporters Buffer Synaptically Released Glutamate on a Submillisecond Time Scale

@article{Diamond1997TransportersBS,
  title={Transporters Buffer Synaptically Released Glutamate on a Submillisecond Time Scale},
  author={Jeffrey S. Diamond and Craig E. Jahr},
  journal={The Journal of Neuroscience},
  year={1997},
  volume={17},
  pages={4672 - 4687}
}
The role of transporters in clearing free glutamate from the synaptic cleft was studied in rat CA1 hippocampal neurons cultured on glial microislands. The time course of free glutamate in the cleft during a synaptic event was estimated by measuring the extent to which the rapidly dissociating AMPA receptor antagonist kynurenate (KYN) was replaced by glutamate during a synaptic response. Dose inhibition of the AMPA receptor EPSC by KYN was less than predicted by the equilibrium affinity of the… Expand
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