Transport properties of toad kidney epithelia in culture.

  title={Transport properties of toad kidney epithelia in culture.},
  author={Frederick M Perkins and Joseph S. Handler},
  journal={The American journal of physiology},
  volume={241 3},
The characteristics of a continuous line of toad kidney epithelial cells (A6) are described. These cells form a monolayer epithelium of high transepithelial electrical resistance (about 5,000 omega . cm2). The cells generate a transepithelial potential difference (apical surface negative) of about 9 mV. The short-circuit current is equivalent to net sodium flux. Net sodium flux is stimulated by aldosterone and by analogues of cAMP. The stimulation is readily reversible. Neither urea… CONTINUE READING