Transport of thiamine and 4-methyl-5-hydroxyethylthiazole by Salmonella typhimurium.

@article{Bellion1983TransportOT,
  title={Transport of thiamine and 4-methyl-5-hydroxyethylthiazole by Salmonella typhimurium.},
  author={E Bellion and Timothy D Lash and B R McKellar},
  journal={Biochimica et biophysica acta},
  year={1983},
  volume={735 3},
  pages={
          331-6
        }
}
The transport of thiamine and 4-methyl-5-hydroxyethylthiazole (MHET), its thiazole moiety, was studied using whole cells of Salmonella typhimurium. It was found that the bacteria possessed an active transport system for thiamine that had Km 0.21 microM and Vmax 33 nmol.min-1.(mg dry wt. cells)-1. Transport of thiamine was glucose dependent, whereas MHET uptake was dependent on both glucose and 2-methyl-4-amino-5-hydroxymethylpyrimidine (MAHMP), the pyrimidine moiety of thiamine. Uptake of both… CONTINUE READING