Transport of plasma membrane‐derived cholesterol and the function of Niemann‐Pick C1 protein

@article{Wiegand2003TransportOP,
  title={Transport of plasma membrane‐derived cholesterol and the function of Niemann‐Pick C1 protein},
  author={Volker Wiegand and Ta-Yuan Chang and Jerome F Strauss and Falk Fahrenholz and Gerald Gimpl},
  journal={The FASEB Journal},
  year={2003},
  volume={17}
}
To visualize the intracellular transport of plasma membrane‐derived cholesterol under physiological and pathophysiological conditions, a novel fluorescent cholesterol analog, 6‐dansyl cholestanol (DChol), has been synthesized. We present several lines of evidence that DChol mimics cholesterol. The cholesterol probe could be efficiently incorporated into the plasma membrane via cyclodextrin‐donor complexes. The itinerary of DChol from the plasma membrane to the cell was studied to determine its… 
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