Transport of methotrexate in L1210 cells: effect of ions on the rate and extent of uptake.

@article{Henderson1980TransportOM,
  title={Transport of methotrexate in L1210 cells: effect of ions on the rate and extent of uptake.},
  author={G. Henderson and E. Zevely},
  journal={Archives of biochemistry and biophysics},
  year={1980},
  volume={200 1},
  pages={
          149-55
        }
}
  • G. Henderson, E. Zevely
  • Published 1980
  • Chemistry, Medicine
  • Archives of biochemistry and biophysics
  • Abstract Transport of methotrexate (MTX) in L1210 cells is highly dependent upon the ionic composition of the external medium. Half-maximal rates of MTX transport ( K t values) vary from 0.9 μ m in cells suspended in potassium-Hepes buffer containing Mg 2+ (Hepes-Mg), to 10 μ m in phosphate-buffered saline (PBS). At saturating levels of substrate, however, transport rates approach the same maximum velocity ( V ) regardless of the buffering medium. The increased K t value for MTX in PBS is due… CONTINUE READING
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