Transport of methotrexate in L1210 cells: effect of ions on the rate and extent of uptake.

@article{Henderson1980TransportOM,
  title={Transport of methotrexate in L1210 cells: effect of ions on the rate and extent of uptake.},
  author={G. Henderson and E. Zevely},
  journal={Archives of biochemistry and biophysics},
  year={1980},
  volume={200 1},
  pages={
          149-55
        }
}
  • G. Henderson, E. Zevely
  • Published 1980
  • Chemistry, Medicine
  • Archives of biochemistry and biophysics
    • Abstract Transport of methotrexate (MTX) in L1210 cells is highly dependent upon the ionic composition of the external medium. Half-maximal rates of MTX transport ( K t values) vary from 0.9 μ m in cells suspended in potassium-Hepes buffer containing Mg 2+ (Hepes-Mg), to 10 μ m in phosphate-buffered saline (PBS). At saturating levels of substrate, however, transport rates approach the same maximum velocity ( V ) regardless of the buffering medium. The increased K t value for MTX in PBS is due… CONTINUE READING
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    References

    SHOWING 1-10 OF 12 REFERENCES
    Antifolate transport in L1210 leukemia cells. Kinetic evidence for the non-identity of carriers for influx and efflux.
    • 38
    Carrier-mediated transport of the folic acid analogue, methotrexate, in the L1210 leukemia cell.
    • 350
    • PDF
    Transport energetics of the folic acid analogue, methotrexate, in L1210 leukemia cells. Enhanced accumulation by metabolic inhibitors.
    • I. Goldman
    • Chemistry, Medicine
    • The Journal of biological chemistry
    • 1969
    • 122
    Photoinactivation of the methotrexate transport system of L1210 cells by 8-azidoadenosin 5'-monophosphate.
    • 19
    Phosphate transport in rat liver mitochondria. Kinetics and energy requirements.
    • 121
    Mechanism of folate transport in Lactobacillus casei: evidence for a component shared with the thiamine and biotin transport systems.
    • 47
    • PDF
    Cyclic adenosine 3':5'-monophosphate and methotrexate transport in L1210 cells.
    • 29
    Membrane transport of antifolates as a critical determinant of drug cytotoxicity.
    • I. Goldman
    • Chemistry, Medicine
    • Advances in experimental medicine and biology
    • 1977
    • 15
    THE CHARACTERISTICS OF THE MEMBRANE TRANSPORT OF AMETHOPTERIN AND THE NATURALLY OCCURRING FOLATES
    • I. Goldman
    • Chemistry, Medicine
    • Annals of the New York Academy of Sciences
    • 1971
    • 287
    Amethopterin transport in Ehrlich ascites carcinoma and L1210 cells.
    • 40
    • PDF