Transport-of-intensity approach to differential interference contrast (TI-DIC) microscopy for quantitative phase imaging.

@article{Kou2010TransportofintensityAT,
  title={Transport-of-intensity approach to differential interference contrast (TI-DIC) microscopy for quantitative phase imaging.},
  author={Shan Shan Kou and Laura Waller and George Barbastathis and Colin J R Sheppard},
  journal={Optics letters},
  year={2010},
  volume={35 3},
  pages={
          447-9
        }
}
Differential interference contrast (DIC) microscopy is an inherently qualitative phase-imaging technique. What is obtained is an image with mixed phase-gradient and amplitude information rather than a true linear mapping of actual optical path length (OPL) differences. Here we investigate an approach that combines the transport-of-intensity equation (TIE) with DIC microscopy, thus improving direct visual observation. There is little hardware modification and the computation is noniterative… CONTINUE READING

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1 Excerpt

DIC image of macrophage cells ( left ) and its reconstructed phase image ( right ) ( Media 1 )

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