Transport of influenza HA from the trans-Golgi network to the apical surface of MDCK cells permeabilized in their basolateral plasma membranes: energy dependence and involvement of GTP-binding proteins

@article{Gravotta1990TransportOI,
  title={Transport of influenza HA from the trans-Golgi network to the apical surface of MDCK cells permeabilized in their basolateral plasma membranes: energy dependence and involvement of GTP-binding proteins},
  author={D. Gravotta and M. Adesnik and D. Sabatini},
  journal={The Journal of Cell Biology},
  year={1990},
  volume={111},
  pages={2893 - 2908}
}
A procedure employing streptolysin O to effect the selective permeabilization of either the apical or basolateral plasma membrane domains of MDCK cell monolayers grown on a filter support was developed which permeabilizes the entire monolayer, leaves the opposite cell surface domain intact, and does not abolish the integrity of the tight junctions. This procedure renders the cell interior accessible to exogenous macromolecules and impermeant reagents, permitting the examination of their effects… Expand
Cell-free reconstitution of the transport of viral glycoproteins from the TGN to the basolateral plasma membrane of MDCK cells.
An in vitro system to study the transport of plasma membrane proteins from the TGN to the basolateral plasma membrane of polarized MDCK cells has been developed in which purified cell fractions areExpand
Basolateral protein transport in streptolysin O-permeabilized MDCK cells
TLDR
The results suggest that the VSV G tail physically interacts with a component of the sorting machinery, and demonstrate the usefulness of the SLO-permeabilized cell system in dissecting the sorting machine. Expand
Rab8, a small GTPase involved in vesicular traffic between the TGN and the basolateral plasma membrane
TLDR
It is concluded that rab8 plays a role in membrane traffic from the TGN to the basolateral plasma membrane in MDCK cells. Expand
ADP-ribosylation Factor 1-independent Protein Sorting and Export from the trans-Golgi Network*
TLDR
The molecular requirements for TGN export of the apical marker influenza hemagglutinin in HeLa cells using an in vitro reconstitution assay suggest that TGN sorting and export of influenza HA does not require classical adaptors involved in the formation of other classes of exocytic carriers and thus appears to proceed via a novel mechanism. Expand
Movement from trans-Golgi network to cell surface in semiintact cells.
TLDR
A method that utilizes semiintact Chinese hamster ovary (CHO) cells to reconstitute vesicular traffic from the trans-Golgi region to the plasma membrane is described, successfully utilized by the group to study intracellular transport in CHO cells. Expand
Regulation of apical transport in epithelial cells by a Gsclass of heterotrimeric G protein
TLDR
It is shown that in the epithelial cell line Madin–Darby Canine Kidney, transport of influenza haemagglutinin protein to the apical surface is stimulated and that of vesicular stomatitis virus glycoprotein to the basolateral surface is retarded by A1F treatment. Expand
Reconstitution of Clathrin‐Independent Endocytosis at the Apical Domain of Permeabilized MDCK II cells: Requirement for a Rho‐Family GTPase
TLDR
Ricin endocytosis in the presence of intact cytosol, as well as GTPγS‐stimulated ricin uptake, was inhibited by Clostridium botulinum C3 transferase, an enzyme found to inactivate Rho proteins, suggesting that one or more of the small GTP binding proteins of the Rho family is involved in regulation of the apical clathrin‐independent endocyTosis in MDCK II cells. Expand
The in Vitro Generation of Post-Golgi Vesicles Carrying Viral Envelope Glycoproteins Requires an ARF-like GTP-binding Protein and a Protein Kinase C Associated with the Golgi Apparatus*
TLDR
The activity of a Golgi-associated protein kinase C was found to be necessary for the release of post-Golgi vesicles, as indicated by the capacity of a variety of inhibitors and antibodies to PKC to suppress it, as well as by the stimulatory effect of the PKC activator 12-O-tetradecanoylphorbol-13-acetate. Expand
Role of heterotrimeric G proteins in polarized membrane transport
TLDR
Results show that the heterotrimeric G proteins differentially regulate the two pathways of polarized transport, and may regulate the process of polarized sorting of proteins in a fashion analogous to their role in signal transduction by providing a communication link with the cytosolic side of the membrane. Expand
Regulation of apical vesicle formation from the trans-Golgi network
TLDR
Comparing HA export to the release of basolateral cargo is compared, showing that nonpolarized cells are capable of differentially sorting distinct classes of cargo into discrete vesicles derived from the TGN, which further the understanding of the regulatory mechanisms underlying apical transmembrane protein export from theTGN. Expand
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TLDR
The intracellular route followed by viral envelope glycoproteins in polarized Madin-Darby canine kidney cells was studied by using temperature-sensitive mutants of vesicular stomatitis virus (VSV) and influenza to suggest that the bulk of HA follows a direct pathway leading from the Golgi to regions of the apical surface close to trans-Golgi cisternae. Expand
Viral glycoproteins destined for apical or basolateral plasma membrane domains traverse the same Golgi apparatus during their intracellular transport in doubly infected Madin-Darby canine kidney cells
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Observations indicate that the site of VSV budding is not determined exclusively by the presence of G polypeptides, and it is likely that, at least for VSV, other cellular or viral components are responsible for the selection of the appropriate budding domain. Expand
A sorting signal for the basolateral delivery of the vesicular stomatitis virus (VSV) G protein lies in its luminal domain: analysis of the targeting of VSV G-influenza hemagglutinin chimeras.
TLDR
Results indicate that information specifying the basolateral transport of the G glycoprotein is located within the first 426 N-terminal amino acids of its ectoplasmic portion. Expand
An enzymatic assay reveals that proteins destined for the apical or basolateral domains of an epithelial cell line share the same late Golgi compartments.
TLDR
It is concluded that most of the newly synthesised basolaterally‐directed G protein is in physical contact with the majority of the neuraminidase through the terminal steps of Golgi processing. Expand
Sorting of an apical plasma membrane glycoprotein occurs before it reaches the cell surface in cultured epithelial cells
TLDR
It is suggested that most newly synthesized hemagglutinin does not transiently appear on the basolateral surface but rather is delivered directly to the apical surface in amounts that account for its final polarized distribution. Expand
Perforated MDCK cells support intracellular transport.
TLDR
The results show that perforated cells provide a convenient and efficient alternative to cell‐free assays for studying the molecular mechanism of intracellular transport and demonstrate that newly synthesized fluorescent sphingolipids were transported from the Golgi complex to the basolateral cell surface in perforation cells. Expand
Microtubule-acting drugs lead to the nonpolarized delivery of the influenza hemagglutinin to the cell surface of polarized Madin-Darby canine kidney cells
TLDR
Since the vesicular stomatitis virus G protein is basolaterally segregated even when the Golgi elements are dispersed and hypothetical tracks disrupted, it appears that the two viral envelope glycoproteins are segregated by fundamentally different mechanisms and that the apical surface may be incapable of accepting vesicles carrying the G protein. Expand
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TLDR
Mechanically perforated MDCK cells were used to study membrane transport between the trans‐Golgi network and the apical and basolateral plasma membrane domains in vitro and control experiments showed that the vesicles were not generated by non‐specific vesiculation of the Golgi complex or the plasma membrane. Expand
Reduced temperature prevents transfer of a membrane glycoprotein to the cell surface but does not prevent terminal glycosylation
TLDR
The transport kinetics of the influenza virus hemagglutinin from its site of synthesis to the apical plasma membrane of Madin-Darby canine kidney cells, a polarized epithelial cell line, were studied by a sensitive tryptic assay, demonstrating that the inhibition at low temperature was reversible. Expand
Exit of newly synthesized membrane proteins from the trans cisterna of the Golgi complex to the plasma membrane
TLDR
The results suggest that the trans cisterna was distinct from the endosome compartment and that the latter was not an obligatory station in the route taken by G protein to the cell surface. Expand
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