Corpus ID: 44656968

Transport of digoxin by human P-glycoprotein expressed in a porcine kidney epithelial cell line (LLC-PK1).

  title={Transport of digoxin by human P-glycoprotein expressed in a porcine kidney epithelial cell line (LLC-PK1).},
  author={Yusuke Tanigawara and N. Okamura and Midori Hirai and Mikiko Yasuhara and Kazumitsu Ueda and Noriyuki Kioka and Tohru Komano and Ryohei Hori},
  journal={The Journal of pharmacology and experimental therapeutics},
  volume={263 2},
This article represents the first evidence that the renal secretion of the commonly used drug, digoxin, is mediated by P-glycoprotein. In this study, it was demonstrated that digoxin is a substrate of P-glycoprotein, and the mechanism of a clinically important drug interaction, such as digoxin-quinidine, was elucidated. Human P-glycoprotein was expressed on the apical membrane of the porcine kidney epithelial cell line, LLC-PK1 by transfecting with human MDR1 cDNA. The expression and function… Expand
Transport of quinolone antibacterial drugs by human P-glycoprotein expressed in a kidney epithelial cell line, LLC-PK1.
It is suggested that quinolone antibacterial drugs are transported by P-glycoprotein, and that P- glycoprotein may contribute at least in part to the renal tubular secretion of quinOLones. Expand
Interaction of Azole Antifungal Agents with Human P-glycoprotein Expressed in a Kidney Epithelial Cell Line, LLC-PK1.
Itraconazole is known to interact with digoxin, a substrate of P-glycoprotein, in clinical situations. In this study, the interactions of the azole antifungal agents, miconazole, fluconazole,Expand
Role of P-glycoprotein in the renal transport of dideoxynucleoside analog drugs.
Data suggest that P-gp does not play a significant role in the renal tubular transport of dideoxynucleoside analog drugs (DADs), and Dipyridamole and dilazep, two nucleoside membrane transport inhibitors, appear to be P- gp inhibitors. Expand
Kinetic Analysis of P-Glycoprotein-Mediated Transport by Using Normal Human Placental Brush-Border Membrane Vesicles
The transport kinetics of transport mediated by Pgp that is physiologically expressed in normal tissue by using human placental brush-border membrane vesicles were successfully evaluated by using BBMVs prepared from normal human placenta. Expand
Renal secretion of xenobiotics mediated by P-glycoprotein: Importance to renal function in health and exploitation for targeted drug delivery to epithelial cysts in polycystic kidney disease
The Madin-Darby canine kidney (MDCK) dog-renal epithelial cell-line is a model for renal tubular P-glycoprotein mediated secretion and a possible role for tubular expression at distal sites in limiting back-diffusion and trapping of moderately lipophilic P- glycoprotein substrates within the renal medulla is suggested. Expand
Effects of P-glycoprotein inhibitors on transepithelial transport of cadmium in cultured renal epithelial cells, LLC-PK1 and LLC-GA5-COL 150.
Results suggest that Cd is extruded from the apical membrane of LLC-PK(1) and LLC-GA5-COL 150 cells, probably depending on the level of P-gp expression. Expand
Directional transcellular transport of bisoprolol in P-glycoprotein-expressed LLC-GA5-COL150 cells, but not in renal epithelial LLC-PK1 Cells.
It is suggested that bisoprolol was not significantly transported via transport systems involved in the directional transport of TEA and cimetidine, but that P-glycoprotein was responsible for the directional Transport of bisoprool as well as quinidine in renal epithelial cells. Expand
Inhibition of P-glycoprotein-mediated drug transport: A unifying mechanism to explain the interaction between digoxin and quinidine [seecomments].
The in vivo data strongly support the hypothesis that inhibition of P-glycoprotein-mediated digoxin elimination plays an important role in the increase of plasma digoxin concentration occurring with quinidine coadministration in wild-type mice and thus support a similar mechanism in humans. Expand
Isolation and characterization of a digoxin transporter and its rat homologue expressed in the kidney.
  • T. Mikkaichi, T. Suzuki, +15 authors T. Abe
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 2004
Human OATP4C1/rat Oatp4c1 might be a first step of the transport pathway of digoxin and various compounds into urine in the kidney, which is one of the most commonly prescribed drugs for the treatment of heart failure. Expand
Effects of P-glycoprotein inhibitors on cadmium accumulation in cultured renal epithelial cells, LLC-PK1, and OK.
Results suggest that P-gp in LLC-PK(1) and OK cells appear to act as an efflux pump of Cd, decreasing cellular Cd accumulation. Expand