Transport of Iron Bound to Recombinant Human Lactoferrin from Rice and Iron Citrate Across Caco-2 Cell Monolayers

@article{Conesa2009TransportOI,
  title={Transport of Iron Bound to Recombinant Human Lactoferrin from Rice and Iron Citrate Across Caco-2 Cell Monolayers},
  author={Celia Conesa and Coloma Pocov{\'i} and Mar{\'i}a D P{\'e}rez and Miguel Calvo Rebollar and Lourdes S{\'a}nchez},
  journal={Bioscience, Biotechnology, and Biochemistry},
  year={2009},
  volume={73},
  pages={2615 - 2620}
}
The possibility of using recombinant human lactoferrin from rice (rhLF) makes it necessary to study its differences from the protein of milk. In this work, the binding of different iron-saturated forms of rhLF to Caco-2 cells was studied. Iron-saturated rhLF bound in higher proportion than the apo-form, but, the data obtained for specific binding were not compatible with receptor-mediated binding. Competition assays showed the same binding capacity for human milk lactoferrin as for rhLF to Caco… 
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Cellular Uptake and Release of Intact Lactoferrin and Its Derivatives in an Intestinal Enterocyte Model of Caco-2 Cells.
TLDR
It is proposed that the uptake and release of intact LF by Caco-2 cells should be assessed as a potential in vitro model of in vivo LF absorption in human intestines.
Involvement of polyamines in iron(III) transport in human intestinal Caco-2 cell lines
TLDR
Iron co-transport in enterocytes, which involved an interaction between iron and polyamine then cell uptake of the polyamine–iron complexes by the polyamines transport system, was more pronounced in proliferating than in differentiated Caco-2 cells, and was observed at physiological concentrations of both polyamines and iron.
Recombinant human lactoferrin: a valuable protein for pharmaceutical products and functional foods.
Lactoferrin’s Anti-Cancer Properties: Safety, Selectivity, and Wide Range of Action
TLDR
Lf shows high bioavailability after oral administration, high selectivity toward cancer cells, and a wide range of molecular targets controlling tumor proliferation, survival, migration, invasion, and metastasization, and was recently found to be an ideal carrier for chemotherapeutics, even for the treatment of brain tumors.
Lactoferrin in the Prevention and Treatment of Intestinal Inflammatory Pathologies Associated with Colorectal Cancer Development
TLDR
This review outlines the most recent studies on lactoferrin as a potential candidate in the prevention and treatment of intestinal inflammatory pathologies that are associated with increased risk of colorectal cancer.

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