Transport of Influenza HA from the trans-Golgi Network to the Apical Surface of MDCK Cells Permeabilized in their Basolateral Plasma Membranes: Energy Dependence and Involvement of GTP-binding Proteins

  • Diego Gravot, David D. Sabat
  • Published 1990


A procedure employing streptolysin O to effect the selective permeabilization of either the apical or basolateral plasma membrane domains of MDCK cell monolayers grown on a filter support was developed which permeabilizes the entire monolayer, leaves the opposite cell surface domain intact, and does not abolish the integrity of the tight junctions. This… (More)


11 Figures and Tables