Transport and phosphorylation of 2-deoxy-D-glucose by amphibian retina. Effects of light and darkness

@article{Witkovsky1982TransportAP,
  title={Transport and phosphorylation of 2-deoxy-D-glucose by amphibian retina. Effects of light and darkness},
  author={Paul Witkovsky and C Y Yang},
  journal={The Journal of General Physiology},
  year={1982},
  volume={80},
  pages={173 - 190}
}
We studied the uptake of 2-deoxy-D-glucose (2DG) and the synthesis of its phosphorylated product 2DG-6-phosphate (2DG-6P) by the retinas of the clawed frog (Xenopus laevis) and the bullfrog (Rana catesbeiana). Autoradiographs showed that most of the retinal 2DG uptake is by the photoreceptor layer. The 2DG accumulation by isolated Xenopus retinas was time and concentration dependent. The Kt for transport was 5.05 mM; Vmax was 6.99 X 10(-10) mol . mg-1 tissue wet weight min-1. The Km for 2DG-6P… CONTINUE READING