Corpus ID: 19509591

Transport and metabolism of methotrexate in normal and resistant cultured rat hepatoma cells.

@article{Galivan1979TransportAM,
  title={Transport and metabolism of methotrexate in normal and resistant cultured rat hepatoma cells.},
  author={J. Galivan},
  journal={Cancer research},
  year={1979},
  volume={39 3},
  pages={
          735-43
        }
}
Abstract The transport and metabolism of methotrexate (MTX) have been examined in monolayer cultures of a cell line derived from the Reuber H35 hepatoma. The H35 cells have a doubling time of approximately 19 hr and cannot grow when MTX is present at 3 × 10 −8 m. The cells exhibit linear uptake of MTX which lasts for 4 to 6 hr and reaches a steady state after approximately 16 hr. The uptake was saturable with a maximal rate of 1.1 nmol/min/g cell protein at an MTX concentration of 18 µm or… Expand

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