Transplantation of the uterus in sheep: Methodology and early reperfusion events

@article{DahmKhler2008TransplantationOT,
  title={Transplantation of the uterus in sheep: Methodology and early reperfusion events},
  author={Pernilla Dahm-K{\"a}hler and Caiza Alm{\'e}n Wranning and Cecilia Lundmark and Anders Enskog and Johan M{\"o}lne and Janusz Marcickiewicz and Randa Racho El-Akouri and JOHN A. McCRACKEN and Mats Br{\"a}nnstr{\"o}m},
  journal={Journal of Obstetrics and Gynaecology Research},
  year={2008},
  volume={34}
}
Aim:  Uterine transplantation is developing into a clinical treatment for uterine factor infertility. An animal model with a similar uterus size and vessels to humans and with pregnancy extending over several months would be beneficial for research on uterine transplantation. The aim of this study was to develop and evaluate autotransplantation of the sheep uterus to an orthotopic position in the pelvis. 
Uterine transplantation: one human case followed by a decade of experimental research in animal models
TLDR
The results of these specific studies on surgical technique, ischaemia‐reperfusion injury, immunosuppression and fertility are discussed.
Test of long‐term uterine survival after allogeneic transplantation in rabbits
  • S. Saso, S. Hurst, +11 authors J Richard Smith
  • Medicine
    The journal of obstetrics and gynaecology research
  • 2014
To see if: (i) a large vessel aortocaval vascular patch technique may bring about long‐term graft survival after allogeneic uterine transplantation (UTn) in a rabbit model; and (ii) fertility can be
First report on fertility after allogeneic uterus transplantation
TLDR
For the first time pregnancy after allogeneic uterus transplantation is described, as a proof of concept of uterine function in a transplanted uterus in a standardized animal model (rat) under immunosuppression.
The development of an extended normothermic ex vivo reperfusion model of the sheep uterus to evaluate organ quality after cold ischemia in relation to uterus transplantation
TLDR
This work aimed to develop an ex vivo sheep uterus reperfusion platform that mimics the reperfusions situation so that initial assessments and comparisons can be performed without the need for costly and labor‐intensive in vivo transplantation experiments.
Uterus Transplantation: An Update and Preparations for Introduction in India
The clinical field of composite tissue transplantation now also includes uterus transplantation (UTx), a new type of quality-oflife enhancing and in this case also life-giving transplantation. The
Uterus transplantation: How far away from human trials?
TLDR
An overview of the animal research that has been conducted in preparation for human uterus transplantation is given and it is predicted that uterus transplants will be introduced as an experimental procedure in the human within a few years.
Achieving uterine auto‐transplantation in a sheep model using iliac vessel anastomosis: a short‐term viability study
To investigate, develop and evaluate anatomical, surgical and anastomotic aspects necessary for a successful uterine transplant in a large‐animal model.
Uterus transplantation: Toward clinical application in Japan
In recent years, uterus transplantation (UTx) has been applied as the treatment for patients with uterine factor infertility worldwide. Thus, the clinical application of UTx in Japan should be
Uterus transplantation in the baboon: methodology and long-term function after auto-transplantation.
TLDR
This study demonstrates the feasibility of UTx by vascular anastomosis in a non-human primate species by developing surgical techniques for uterus retrieval and transplantation in the baboon.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 36 REFERENCES
Auto‐transplantation of the uterus in the domestic pig (Sus scrofa): Surgical technique and early reperfusion events
Aim:  To develop a method for auto‐transplantation of the uterus in the pig and to evaluate the early reperfusion events after short‐term cold ischemia.
Transplantation of the uterus
Successful uterine transplantation in the mouse: pregnancy and post-natal development of offspring.
TLDR
These observations document the capacity of a transplanted uterus to harbour pregnancies to term, and reveal that offspring from a transplants uterus develop to normal fertile adults.
Heterotopic uterine transplantation by vascular anastomosis in the mouse.
TLDR
An undisturbed function of the transplanted uterus horn was finally demonstrated by its ability to implant inserted blastocysts and to carry pregnancy with fetal weight being similar to that of fetuses in the native uterus and controls.
Transplantation of the human uterus
  • W. Fageeh, H. Raffa, H. Jabbad, A. Marzouki
  • Medicine
    International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics
  • 2002
Replantation of uterus and ovaries in dogs, with successful pregnancy.
TLDR
These studies apparently represent the first in which vascular anastomoses were used for successful uterine replantation and were supported by aortography, hysterography, studies of vaginal epithelium, and evidence of ovulation.
Preliminary results of orthotopic en bloc uterus and ovary transplantation in the laboratory rat.
TLDR
Uterus transplantation in rats has proven to be a valid experiment that allows us to express hope that by further research on transplantation of the uterus gynecologists will be able to introduce an adapted technique in the treatment of specific cases of human female infertility.
Rejection patterns in allogeneic uterus transplantation in the mouse.
TLDR
A murine model to study rejection of allogeneic uterus transplants was characterized and signs of rejection were seen from day 2 to day 5 and severe rejection was seen from days 10 to day 15, useful in future studies on immunosuppressants in this model.
Short-term ischaemic storage of human uterine myometrium--basic studies towards uterine transplantation.
TLDR
The results show that human uterine myometrial tissue is resistant towards cold ischaemia for at least 6 h if stored in UW and PER solutions.
...
1
2
3
4
...