Transplantation of purified islet cells in diabetic rats. I. Standardization of islet cell grafts.

@article{Pipeleers1991TransplantationOP,
  title={Transplantation of purified islet cells in diabetic rats. I. Standardization of islet cell grafts.},
  author={Daniel G. Pipeleers and Miriam A. Pipeleers-Marichal and Jean Claude Hannaert and M C Berghmans and P A in't Veld and Jan Rozing and M van de Winkel and Willy Gepts},
  journal={Diabetes},
  year={1991},
  volume={40 7},
  pages={908-19}
}
A standardized procedure was developed for the preparation of rat islet cell grafts with selected cell number and composition. After collagenase digestion of pancreases and elutriation of tissue fragments, islets were isolated and dissociated, and cells were purified by autofluorescence-activated cell sorting. Approximately 30% of the initial beta-cell mass was lost during digestion and elimination of small mostly exocrine particles. Fifty percent was recovered in isolated islet preparations… CONTINUE READING

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