Transplantation of human embryonic stem cell-derived photoreceptors restores some visual function in Crx-deficient mice.

@article{Lamba2009TransplantationOH,
  title={Transplantation of human embryonic stem cell-derived photoreceptors restores some visual function in Crx-deficient mice.},
  author={Deepak A Lamba and Juliane Gust and Thomas A. Reh},
  journal={Cell stem cell},
  year={2009},
  volume={4 1},
  pages={
          73-9
        }
}
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...

References

SHOWING 1-10 OF 26 REFERENCES

Efficient generation of retinal progenitor cells from human embryonic stem cells

It is demonstrated that human ES cells can be selectively directed to a neural retinal cell fate and thus may be useful in the treatment of retinal degenerations.

Multipotent retinal progenitors express developmental markers, differentiate into retinal neurons, and preserve light-mediated behavior.

A subset of the retinal progenitor cells developed into mature neurons, including presumptive photoreceptors expressing recoverin, rhodopsin, or cone opsin, in mice with retinal degeneration.

Retinal Incorporation and Differentiation of Neural Precursors Derived from Human Embryonic Stem Cells

The results suggest that hESCs have the potential to differentiate into retinal cells and that the subretinal microenvironment supports their differentiation toward a photoreceptor fate.

Toward the generation of rod and cone photoreceptors from mouse, monkey and human embryonic stem cells

In vitro generation of putative rod and cone photoreceptors from mouse, monkey and human ES cells by stepwise treatments under defined culture conditions, in the absence of retinal tissues, may facilitate the development of human ES cell–based transplantation therapies for retinal diseases.

Widespread Integration and Survival of Adult-Derived Neural Progenitor Cells in the Developing Optic Retina

Adult rat hippocampus-derived neural progenitor cells (AHPC) show considerable adaptability following grafting to several brain regions and adopt the morphologies and positions of Müller, amacrine, bipolar, horizontal, photoreceptor, and astroglial cells, suggesting that the adult-derived stem cells can adapt to a wide variety of heterologous environments.

In Vitro Isolation and Expansion of Human Retinal Progenitor Cells

Findings indicate that human retina at the 10th-13th week of gestation harbors progenitor cells that can be maintained and expanded in vitro for multiple generations, and may have important implications with respect to human degenerative retinal diseases.

Facile isolation and the characterization of human retinal stem cells.

The transplanted dissociated human retinal sphere cells, containing both stem cells and progenitors, into the eyes of postnatal day 1 NOD/SCID mice and embryonic chick eyes were able to survive, migrate, integrate, and differentiate into the neural retina, especially as photoreceptors.

Regulation of proliferation and photoreceptor differentiation in fetal human retinal cell cultures.

Fetal human retinal cells can be maintained and expanded in vitro, indicating that this technique may be useful for generating large numbers of Retinal cells.

Neural regeneration and cell replacement: a view from the eye.

Development and degeneration of retina in rds mutant mice: The electroretinogram