Transplantation of Chondrocytes Utilizing a Polymer‐Cell Construct to Produce Tissue‐Engineered Cartilage in the Shape of a Human Ear

  title={Transplantation of Chondrocytes Utilizing a Polymer‐Cell Construct to Produce Tissue‐Engineered Cartilage in the Shape of a Human Ear},
  author={Yilin Cao and Joseph Phillip Vacanti and Keith T. Paige and Joseph Upton and Charles A. Vacanti},
  journal={Plastic and Reconstructive Surgery},
&NA; This study evaluates the feasibility of growing tissue‐engineered cartilage in the shape of a human ear using chondrocytes seeded onto a synthetic biodegradable polymer fashioned in the shape of a 3‐year‐old child's auricle. A polymer template was formed in the shape of a human auricle using a nonwoven mesh of polyglycolic acid molded after being immersed in a 1% solution of polylactic acid. Each polyglycolic acid‐polylactic acid template was seeded with chondrocytes isolated from bovine… 

Tissue engineering a model for the human ear: Assessment of size, shape, morphology, and gene expression following seeding of different chondrocytes

  • H. KusuharaN. Isogai W. Landis
  • Biology
    Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society
  • 2009
The collective data suggest that nasoseptal, articular, and auricular cartilages represent harvest sites suitable for development of tissue‐engineered human ear models with retention over time of three‐dimensional construct architecture, gene expression, and extracellular matrix composition comparable to normal, nonmineralizing cartilage.

Tissue engineering of an auricular cartilage model utilizing cultured chondrocyte-poly(L-lactide-epsilon-caprolactone) scaffolds.

It is demonstrated that poly(L-lactic acid-epsilon-caprolactone) copolymer seeded with articular chondrocytes supports development and maintenance of cartilage in a human ear shape over periods to 40 weeks in this implantation model.

Tissue Engineering of Autologous Cartilage for Craniofacial Reconstruction by Injection Molding

The goal of this study was to develop a simple method to create complex structures with good three-dimensional tolerance in order to form cartilage in specific shapes in an autologous animal model.

Tissue‐Engineered Nipple Reconstruction

A biodegradable, biocompatible copolymer of polyethylene oxide and polypropylene oxide (Pluronic F‐127), which exists as a liquid below 4°C and polymerizes to a thick gel when it is exposed to physiologic temperatures (body temperatures), was used as a vehicle for chondrocyte delivery and as a scaffold to guide growth.

Tissue-Engineered Flexible Ear-Shaped Cartilage

This study demonstrates that it is possible to engineer a cartilage construct that resembles the human ear not only in shape but also in size and flexibility and confirms that lamination is a reliable method to confer elastic-like flexibility to an engineered cartilage Construct.

Engineering ear constructs with a composite scaffold to maintain dimensions.

It is hypothesized that a permanent support in the form of a coiled wire embedded into a porous collagen scaffold will maintain the construct's size and ear-specific shape and there were no adverse effects on neocartilage formation from the embedded wire.

Bioengineering of elastic cartilage with aggregated porcine and human auricular chondrocytes and hydrogels containing alginate, collagen, and kappa-elastin.

Modification of the basic techniques that lead to production of a large amount of elastic cartilage originated from porcine and human isolated chondrocytes are described, which closely resembled native auricular cartilage at the gross, microscopic, and ultrastructural levels.

Novel approach to engineer implantable nasal alar cartilage employing marrow precursor cell sheet and biodegradable scaffold.

  • Junrui ZhangLiangqi Liu T. Mao
  • Medicine, Biology
    Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons
  • 2009



Synthetic Polymers Seeded with Chondrocytes Provide a Template for New Cartilage Formation

The delivery of chondrocytes on synthetic polymers configured to provide a large surface area for cell attachment and thus to allow cell function and survival by diffusion of nutrients has resulted in the creation of macroscopic plates of up to 100 mg of new cartilage subcutaneously in 19 of 21 animals.

Large-scale preparation of chondrocytes.

Reconstruction of the Burned External Ear Using a Medpor Porous Polyethylene Pivoting Helix Framework

  • T. Wellisz
  • Medicine
    Plastic and reconstructive surgery
  • 1993
A new technique using a Medpor porous polyethylene pivoting helix framework for reconstruction of the burned auricle restored an ear that was both aesthetically pleasing and functional so as to provide support for a pair of eyeglasses.

Osseointegrated Alloplastic versus Autogenous Ear Reconstruction: Criteria for Treatment Selection

The main indications for autogenous reconstruction include classical microtia, relatively normal lower one-third of the ear, patient preference, and less compliant patients.

Auricular Repair with Autogenous Rib Cartilage Grafts: Two Decades of Experience with 600 Cases

  • B. Brent
  • Medicine
    Plastic and reconstructive surgery
  • 1992
A sound approach to auricular construction using methods that have evolved through two decades of personal experience with 600 cases is presented and pertinent information I have gleaned from a questionnaire sent to my operated patients is discussed.

Refinements in pediatric microtia reconstruction.

  • R. EaveyD. Ryan
  • Medicine
    Archives of otolaryngology--head & neck surgery
  • 1996
Surgical techniques that address the problems of donor sites for the rib harvest and for subsequent skin grafting and an unexpected shortage of rib can be encountered in some children.