Transmitter localization and vesicle turnover at a serotoninergic synapse between identified leech neurons in culture

@article{Kuffler1987TransmitterLA,
  title={Transmitter localization and vesicle turnover at a serotoninergic synapse between identified leech neurons in culture},
  author={Damien P. Kuffler and John G. Nicholls and Pierre Drapeau},
  journal={Journal of Comparative Neurology},
  year={1987},
  volume={256}
}
1 An electron microscopic study has been made of chemical synapses that develop between identified nerve cells isolated from the CNS of the leech and maintained in culture. Structures resembling synapses were observed in pairs of Retzius cells and P sensory cells at which chemical transmission had been demonstrated by recording with microelectrodes. Vesicle recycling was shown by following the uptake of extracellular markers after stimulation. 2 The membrane separation between the presynaptic… 
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    Proceedings of the Royal Society of London. B. Biological Sciences
  • 1989
TLDR
The results show that the tip of a neuron represents a preferential site for the formation of chemical synapses in leech neurons in culture, and a marked difference was in the speed and reliability with which chemicalsynapses developed when stumps were in contact.
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It is concluded that innervation of a P cell by a Retzius cell resulted in clustering of the synaptic 5-HT receptors, which activate Cl channels and reduction of the nonsynaptic, cationic response.
Contact-mediated loss of the nonsynaptic response to transmitter during reinnervation of an identified leech neuron in culture
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The results demonstrate that the loss of the nonsynaptic response of the P cell to transmitter is due to contact between the neurons rather than to the release of transmitter or trophic factors.
Biophysics of Active Vesicle Transport, an Intermediate Step That Couples Excitation and Exocytosis of Serotonin in the Neuronal Soma
TLDR
It is confirmed that active vesicle transport is an intermediate step for somatic serotonin exocytosis by Retzius neurons and provides a quantitative method for analyzing similar phenomena in other cell types.
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