Transmission electron microscopic studies of stages of Histomonas meleagridis from clonal cultures

@article{Mielewczik2008TransmissionEM,
  title={Transmission electron microscopic studies of stages of Histomonas meleagridis from clonal cultures},
  author={Michael Mielewczik and Heinz Mehlhorn and Saleh A S Al-Quraishy and Elvira Grabensteiner and Michael Hess},
  journal={Parasitology Research},
  year={2008},
  volume={103},
  pages={745-750}
}
Histomonas meleagridis is a 10–20 μm-sized flagellated protozoan, causing histomoniasis in gallinaceous birds. Different strains of H. meleagridis from different origins were used to establish clonal cultures, which can be traced back to a single cell. Cells from these clonal cultures were examined by transmission electron microscopy. Results gave detailed insights in the ultrastructure showing a single flagellum, a band of microtubules remnants of an axostyle or of a costa, respectively pelta… Expand
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A microscopic description and ultrastructural characterization 1 of Dientamoeba fragilis : an emerging cause of human enteric 2 disease 3 4 5
1 2 Dientamoeba fragilis is a pathogenic trichomonad found in the gastrointestinal tract of 3 humans and is implicated as a cause of diarrhoea. Despite its discovery over a century 4 ago, there hasExpand
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References

SHOWING 1-10 OF 21 REFERENCES
Clonal cultures of Histomonas meleagridis, Tetratrichomonas gallinarum and a Blastocystis sp. established through micromanipulation
Clonal cultures of Histomonas meleagridis, Tetratrichomonas gallinarum and a Blastocystis sp. were established for the first time. Single microbes were successfully isolated from a mixture ofExpand
Histomonas meleagridis and several bacteria as agents of infectious enterohepatitis in gnotobiotic turkeys.
TLDR
A dual etiology, involving a protozoan and a single species of bacteria, has been demonstrated for infectious enterohepatitis of turkeys using gnotobiotic (“bacteria-free”) turkeys, and Koch's postulates for suspected disease organisms were fulfilled. Expand
The structure and development of Histomonas meleagridis (Mastigamoebidae: Protozoa) in the female reproductive tract of its intermediate host, Heterakis gallinarum (Nematoda).
TLDR
There is strong circumstantial evidence that the nematode is able to repair damage to the wall of the reproductive system and to the oolemma caused by the protozoan. Expand
Hydrogenosome behavior during the cell cycle in Tritrichomonas foetus
TLDR
The hydrogenosome behavior in the protist Tritrichomonas foetus, parasite of the urogenital tract of cattle, is reported here and similarities between hydrogenosomes and mitochondria behavior during the cell cycle are discussed. Expand
Blackhead Disease (Histomoniasis) in Poultry: A Critical Review
TLDR
It was demonstrated that histomoniasis could spread rapidly through a flock of turkeys by direct contact, probably involving the phenomenon of cloacal drinking, and the lack of suitable treatment drugs or vaccines emphasizes the importance of prevention by worm control and management. Expand
Histomoniasis in gnotobiotic chickens and turkeys: biological aspects of the role of bacteria in the etiology.
TLDR
The role of bacteria apparently was more vital for the survival of Heterakis than Histomonas in the pathogenesis of infectious enterohepatitis and the essential contributing factor was determined to be neither a favorable pH nor an oxidation-reduction potential value within the intestine of the host. Expand
Lymphocytes of the intestinal mucosa of chickens
TLDR
The granulated cells were identical to those previously designated globule-containing leukocytes, but were distinct from mast cells in their morphology, staining reactions and the stability of the granules in different fixatives and buffers. Expand
Effects of imidacloprid on adult and larval stages of the flea Ctenocephalides felis after in vivo and in vitro application: a light- and electron-microscopy study
TLDR
It is shown that imidacloprid kills larval and adult flea stages rapidly via the same mode of action and thus prevents the development of flea populations in human or animal dwellings. Expand
Contribution of cryofixation and freeze‐substitution to analytical microscopy: A study of Tritrichomonas foetus hydrogenosomes
TLDR
A comparative microanalytical study of different fixation methods for electron microscopy analyzing hydrogenosomes of the bovine parasite Tritrichomonas foetus found that the ultrastructural preservation achieved by the cryotechnique was far superior to the chemical fixation, since it allowed the successful cryoimmobilization of intracellular ion contents. Expand
Tritrichomonas foetus: ultrastructural localization of calcium in the plasma membrane and in the hydrogenosome.
TLDR
Results suggest that the hydrogenosome may be involved in the control of the intracellular concentration of Ca 2+ in T. foetus. Expand
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