Transmembrane beta-barrel of staphylococcal alpha-toxin forms in sensitive but not in resistant cells.

@article{Valeva1997TransmembraneBO,
  title={Transmembrane beta-barrel of staphylococcal alpha-toxin forms in sensitive but not in resistant cells.},
  author={Angela Valeva and Iwan Walev and M Pinkernell and Brian Walker and Hagan Bayley and Michael Palmer and Sucharit Bhakdi},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1997},
  volume={94 21},
  pages={11607-11}
}
Staphylococcal alpha-toxin is a 293-residue, single-chain polypeptide that spontaneously assembles into a heptameric pore in target cell membranes. To identify the pore-forming domain, substitution mutants have been produced in which single cysteine residues were introduced throughout the toxin molecule. By attaching the environmentally sensitive dye acrylodan to the sulfhydryl groups, the environment of individual amino acid side chains could be probed. In liposomes, a single 23-amino acid… CONTINUE READING

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