Translocation of the glucose transporter (GLUT4) to the cell surface in permeabilized 3T3-L1 adipocytes: effects of ATP insulin, and GTP gamma S and localization of GLUT4 to clathrin lattices

@article{Robinson1992TranslocationOT,
  title={Translocation of the glucose transporter (GLUT4) to the cell surface in permeabilized 3T3-L1 adipocytes: effects of ATP insulin, and GTP gamma S and localization of GLUT4 to clathrin lattices},
  author={Lisa J. Robinson and Suhong Pang and Dr. Stanley Harris and John M Heuser and David E. James},
  journal={The Journal of Cell Biology},
  year={1992},
  volume={117},
  pages={1181 - 1196}
}
Insulin stimulates the movement of two glucose transporter isoforms (GLUT1 and GLUT4) to the plasma membrane (PM) in adipocytes. To study this process we have prepared highly purified PM fragments by gently sonicating 3T3-L1 adipocytes grown on glass coverslips. Using confocal laser immunofluorescence microscopy we observed increased PM labeling for GLUT1 (2.3-fold) and GLUT4 (eightfold) after insulin treatment in intact cells. EM immunolabeling of PM fragments indicated that in the… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 67 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 28 references

Translocation of the glucose transporter GLUT4 in cardiac myocytes of the rat

J. W. Slot, H. J. Cmuze, S. Gigangack, D. E. James, G. E. Lienhard.
Proc. Natl. Acad. Sci. USA. 88:7815-7819. • 1991
View 9 Excerpts
Highly Influenced

Clathrin, adaptors, and sorting.

Annual review of cell biology • 1990
View 1 Excerpt

Similar Papers

Loading similar papers…