Translational repression and specific RNA binding by the coat protein of the Pseudomonas phage PP7.

@article{Lim2001TranslationalRA,
  title={Translational repression and specific RNA binding by the coat protein of the Pseudomonas phage PP7.},
  author={Francis Pol C. Lim and Thomas Downey and David S. Peabody},
  journal={The Journal of biological chemistry},
  year={2001},
  volume={276 25},
  pages={22507-13}
}
PP7 is a single-strand RNA bacteriophage of Pseudomonas aeroginosa and a distant relative to coliphages like MS2 and Qbeta. Here we show that PP7 coat protein is a specific RNA-binding protein, capable of repressing the translation of sequences fused to the translation initiation region of PP7 replicase. Its RNA binding activity is specific since it represses the translational operator of PP7, but does not repress the operators of the MS2 or Qbeta phages. Conditions for the purification of coat… CONTINUE READING
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