Translational efficiency of polycistronic mRNAs and their utilization to express heterologous genes in mammalian cells.

@article{Kaufman1987TranslationalEO,
  title={Translational efficiency of polycistronic mRNAs and their utilization to express heterologous genes in mammalian cells.},
  author={Randal J Kaufman and P E Murtha and Monique V Davies},
  journal={The EMBO journal},
  year={1987},
  volume={6 1},
  pages={187-93}
}
The translation of polycistronic mRNAs in mammalian cells was studied. Transcription units, constructed to contain one, two or three open reading frames (ORFs), were introduced stably into Chinese hamster ovary cells and transiently into COS monkey cells. The analysis of mRNA levels and protein synthesis in these cells demonstrated that the mRNAs… CONTINUE READING