Translational control in heat-shocked Drosophila embryos. Evidence for the inactivation of initiation factor(s) involved in the recognition of mRNA cap structure.

@article{Maroto1988TranslationalCI,
  title={Translational control in heat-shocked Drosophila embryos. Evidence for the inactivation of initiation factor(s) involved in the recognition of mRNA cap structure.},
  author={Federico Garc{\'i}a Maroto and Jos{\'e} Manuel Sierra},
  journal={The Journal of biological chemistry},
  year={1988},
  volume={263 30},
  pages={
          15720-5
        }
}
Translational Control in Heat-shocked Drosophila Embryos
TLDR
Data are consistent with the notion that the failure of normal mRNAs to be translated under heat shock conditions might be due, at least to some extent, to the inactivation of polypeptide chain initiation factor(s) involved in the recognition of the mRNA 5”terminal cap structure.
embryos Drosophila mRNAs in heat shock protein 70 and reaper Internal ribosome entry site drives cap-independent translation of
TLDR
In vitro translation experiments using wild-type and eIF4E mutant embryonic extracts show that reporter mRNA bearing reaper 5 untranslated region (UTR) is effectively translated in a cap-independent manner.
Internal ribosome entry site drives cap-independent translation of reaper and heat shock protein 70 mRNAs in Drosophila embryos.
TLDR
In vitro translation experiments using wild-type and eIF4E mutant embryonic extracts show that reporter mRNA bearing reaper 5' untranslated region (UTR) is effectively translated in a cap-independent manner.
m7GpppG cap dependence for efficient translation of Drosophila 70-kDa heat-shock-protein (Hsp70) mRNA.
TLDR
Complementary experiments in which eIF-4 was inactivated in vitro using either m7GTP cap analogue or foot-and-mouth-disease virus L protease expression indicated that the cap-dependent translation pathway is required for optimal Hsp mRNA translation.
Cap-independent translation of heat shock messenger RNAs.
TLDR
It has now become apparent that there are numerous similarities between translation in heat-shocked cells and translation in picornavirus-infected cells and other cells which predominately carry out cap-independent translation.
Heat Shock Disrupts Cap and Poly(A) Tail Function during Translation and Increases mRNA Stability of Introduced Reporter mRNA
TLDR
Message stability increased as a function of the severity of the heat shock so that following a mild to moderate stress the increase in message stability more than compensated for the reduction in cap and poly(A) tail function.
Sequence and structure determinants of Drosophila Hsp70 mRNA translation: 5'UTR secondary structure specifically inhibits heat shock protein mRNA translation.
TLDR
Results indicate that heat shock reduces the capacity to unwind 5-UTR secondary structure, allowing only mRNAs with minimal 5'-UTRsecondary structure to be efficiently translated.
Efficient translation of an SSA1-derived heat-shock mRNA in yeast cells limited for cap-binding protein and eIF-4F
TLDR
It is reported here that decreased global translation initiation in cdc33 mutant cells has virtually no effect on the translation of mRNA from the SSA1 -lacZ chimeric gene, comprised of yeast SSA 1 hsp70 gene transcription and translation initiation sequences fused in-frame to the bacterial lacZ gene.
Transcriptional regulation in Drosophila during heat shock: A nuclear run-on analysis
TLDR
A nuclear run-on assay was used as a novel approach to study the changes in transcriptional activity that take place in Drosophila melanogaster during heat shock, and different heat shock genes were transcribed at strikingly different rates, which varied over a 20-fold range.
Translational regulation of the heat shock response
TLDR
The emerging picture is that the two key steps of polypeptide chain initiation, namely mRNA binding and Met-tRNAi binding to ribosomes, are regulated in heat-shocked mammalian cells.
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