Translational activity of messenger ribonucleic acid isolated from unstimulated and phytohaemagglutinin-activated lymphocytes.

@article{Wallace1979TranslationalAO,
  title={Translational activity of messenger ribonucleic acid isolated from unstimulated and phytohaemagglutinin-activated lymphocytes.},
  author={Donald M. Wallace and Rosemary Jagus and C R Benzie and John E. Kay},
  journal={The Biochemical journal},
  year={1979},
  volume={184 2},
  pages={
          277-82
        }
}
Purified cytoplasmic poly(A)+ RNA isolated from unstimulated pig lymphocytes has the same ability to direct translation in a range of cell-free systems as the corresponding mRNA from 20h phytohaemagglutinin-activated lymphocytes. Additional methylation of the mRNA is not required for maximum protein synthesis in the wheat-germ cell-free system. Misleading results are obtained if the mRNA preparations used are not adequately purified, and a method suitable for routine assessment of the degree of… CONTINUE READING
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