• Corpus ID: 10971826

Translational Control in Heat-shocked Drosophila Embryos

  title={Translational Control in Heat-shocked Drosophila Embryos},
  author={Federico G. MarotoS and Jos{\'e} Manuel Sierra},
Lysates from normally growing (25 “C) or heatshocked (37 “C, 45 min) Drosophila melanogaster embryos were obtained and the effect of analogues of the mRNA 5”terminal cap, m7G(5’)ppp(5’)N structure and of potassium ions on their endogenous protein synthesis activity was studied. At optimal concentration of KCHsCOO (75-80 mM), protein synthesis in normal lysates is strongly inhibited by cap analogues (m7GpppG, m7GDP, and m’GMP). At the same ionic conditions, heat shock lysates translate… 
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