Translation of messenger RNA from canine tracheal epithelial cells: identification of mucin core protein.

@article{Virmani1991TranslationOM,
  title={Translation of messenger RNA from canine tracheal epithelial cells: identification of mucin core protein.},
  author={Arvind K. Virmani and Vepatu Shankar and Michael S. Gilmore and Donald C. Graves and Goverdhan P. Sachdev},
  journal={American journal of respiratory cell and molecular biology},
  year={1991},
  volume={5 2},
  pages={149-54}
}
A high-molecular-weight mucin (Mr approximately 11.0 x 10(6)) was purified from canine tracheal pouch secretions. The mucin was deglycosylated by treatment with trifluoromethane sulfonic acid for 8 h at 8 degrees C and subsequently with alpha-N-acetylgalactosaminidase. These treatments almost completely removed the carbohydrate moieties. The amino acid compositions of the deglycosylated and native mucins were similar, indicating that the deglycosylation procedure used did not cause notable… CONTINUE READING