Translation of mRNA from phytohemagglutinin-stimulated human lymphocytes: characterization of interferon mRNAs.

@article{Vaquero1982TranslationOM,
  title={Translation of mRNA from phytohemagglutinin-stimulated human lymphocytes: characterization of interferon mRNAs.},
  author={Catherine Vaquero and Josiane Sanc{\'e}au and L Catinot and Georges Andreu and E T Falcoff and R Falcoff},
  journal={Journal of interferon research},
  year={1982},
  volume={2 2},
  pages={217-28}
}
Human lymphocytes obtained by cytapheresis were stimulated in spinner culture conditions by nonpurified PHA in order to study the production of gamma interferon, and the characterization of IFN-gamma mRNA. Titers of interferon prepared in 0.6 to 4 1 batches, varied in 20 preparations from 8,000 to 32,000 units/ml. This interferon was unstable at pH 2: the residual antiviral activity after 20 h treatment was less than 3%. Antibodies raised against gamma interferon from Con A and SEA-stimulated… CONTINUE READING