Translation initiation factor 3 antagonizes authentic start codon selection on leaderless mRNAs.

@article{Tedin1999TranslationIF,
  title={Translation initiation factor 3 antagonizes authentic start codon selection on leaderless mRNAs.},
  author={Karsten Tedin and Isabella Moll and Sonja Grill and Armin Resch and Anton Graschopf and Claudio O. Gualerzi and Udo Blaesi},
  journal={Molecular microbiology},
  year={1999},
  volume={31 1},
  pages={67-77}
}
In this study, we have examined the influence of initiation factors on translation initiation of leaderless mRNAs whose 5'-terminal residues are the A of the AUG initiating codon. A 1:1 ratio of initiation factors to ribosomes abolished ternary complex formation at the authentic start codon of different leaderless mRNAs. Supporting this observation, in vitro translation assays using limiting ribosome concentrations with competing leaderless lambda cl and Escherichia coli ompA mRNAs, the latter… CONTINUE READING