Translation inhibitors cause abnormalities in ribosome profiling experiments

@inproceedings{Gerashchenko2014TranslationIC,
  title={Translation inhibitors cause abnormalities in ribosome profiling experiments},
  author={Maxim V. Gerashchenko and Vadim N. Gladyshev},
  booktitle={Nucleic acids research},
  year={2014}
}
Ribosome profiling and high-throughput sequencing provide unprecedented opportunities for the analysis of mRNA translation. Using this novel method, several studies have demonstrated the widespread role of short upstream reading frames in translational control as well as slower elongation at the beginning of open reading frames in response to stress. Based on the initial studies, the importance of adding or omitting translation inhibitors, such as cycloheximide, was noted as it markedly… CONTINUE READING
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