Translation in Saccharomyces cerevisiae: initiation factor 4E-dependent cell-free system.

@article{Altmann1989TranslationIS,
  title={Translation in Saccharomyces cerevisiae: initiation factor 4E-dependent cell-free system.},
  author={Michael Altmann and Nahum Sonenberg and H. Trachsel},
  journal={Molecular and cellular biology},
  year={1989},
  volume={9 10},
  pages={4467-72}
}
The gene encoding translation initiation factor 4E (eIF-4E) from Saccharomyces cerevisiae was randomly mutagenized in vitro. The mutagenized gene was reintroduced on a plasmid into S. cerevisiae cells having their only wild-type eIF-4E gene on a plasmid under the control of the regulatable GAL1 promoter. Transcription from the GAL1 promoter (and consequently the production of wild-type eIF-4E) was then shut off by plating these cells on glucose-containing medium. Under these conditions, the… CONTINUE READING

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