Transiently Expressed Mistletoe Lectin II in Nicotiana benthamiana Demonstrates Anticancer Activity In Vitro

@article{Mazalovska2020TransientlyEM,
  title={Transiently Expressed Mistletoe Lectin II in Nicotiana benthamiana Demonstrates Anticancer Activity In Vitro},
  author={Milena Mazalovska and J. C. Kouokam},
  journal={Molecules},
  year={2020},
  volume={25}
}
Mistletoe (Viscum album) extracts have been used as alternative and complementary therapeutic preparations in multiple cancers for decades. Mistletoe lectins (ML-I, ML-II, and ML-III) are considered to be the main anticancer components of such preparations. In the present study, ML-II was transiently expressed in Nicotiana benthamiana using the pEAQ-HT expression system. Expression levels of up to 60 mg/kg of the infiltrated plant tissue were obtained, and a three-fold increase was achieved by… Expand
1 Citations
Mistletoe lectins: From interconnecting proteins to potential tumour inhibiting agents
TLDR
This review aims at exploring the antitumor mechanisms of MLs against various cancer cell lines and mice animal models which encourages its application as effective bio-therapeutic tools to address range of human cancers. Expand

References

SHOWING 1-10 OF 46 REFERENCES
Characterization of recombinant and plant-derived mistletoe lectin and their B-chains.
TLDR
It is concluded that both, lectin activity and the rRNA N-glycosidase activity, are prerequisites for the cytotoxic effects on target cells. Expand
Molecular mechanisms of mistletoe plant extract-induced apoptosis in acute lymphoblastic leukemia in vivo and in vitro.
TLDR
The in vivo efficacy and mechanism of action of MT in lymphoblastic leukemia is described for the first time and DNA-fragmentation assays indicated that dose-dependent induction of apoptosis was the main mechanism of cell death. Expand
Isolation and characterization of viscumin, a toxic lectin from Viscum album L. (mistletoe).
TLDR
Gel filtration experiments on Sephacryl 200 indicated that, at low concentrations, viscumin occurs as a monomer and at higher concentrations as a dimer, and protection experiments with antiserum against viscumin indicated that the major part of the cytotoxic activity in mistletoe extracts is due to viscumin. Expand
Modulation of cytotoxicity and enhancement of cytokine release induced by Viscum album L. extracts or mistletoe lectins
TLDR
The experimental results are discussed in relation to the treatment of cancer patients administered with mistletoe extracts and lectin cytotoxicity, which enhanced the production of cytokines by isolated human monocytes. Expand
Apoptosis-inducing properties of Viscum album L. extracts from different host trees, correlate with their content of toxic mistletoe lectins.
TLDR
Although the mistletoe lectin (ML) content of VAL extracts strongly correlated with their apoptosis-inducing properties, the presence of these proteins will not guarantee its biological activity, indicating the involvement of other components which may modulate ML cytotoxicity. Expand
The potent anti-cancer activity of Dioclea lasiocarpa lectin.
TLDR
Interestingly, DLasiL lectin caused a G2/M arrest in A2780 cells after 24h exposure, activating caspase 9 and delaying the on-set of apoptosis, and it is suggested that further investigations to explore the potential ofDLasiL as a new anticancer agent are warranted. Expand
Combined cytotoxic action of Viscum album agglutinin-1 and anticancer agents against human A549 lung cancer cells.
TLDR
The findings suggest that the simultaneous administration of VAA-1 with all anticancer agents tested is advantageous since cytotoxic effects are enhanced. Expand
The cytotoxic effect of mistletoe lectins I, II and III on sensitive and multidrug resistant human colon cancer cell lines in vitro.
TLDR
Higher sensitivity of HT 29(mdr+) cells are not caused by the overexpression of MDR-1, but by the general changes of the cellular glycosylation during the acquisition of the MDR phenotype. Expand
Isolation and properties of three lectins from mistletoe (Viscum album L.).
TLDR
Three lectins have been isolated from an extract of mistletoe by affinity chromatography on partially hydrolysed Sepharose and human immunoglobulin- Separose and all three react with human erythrocytes without specificity for the A, B, and O blood groups. Expand
Lectin from Dioclea violacea induces autophagy in U87 glioma cells.
TLDR
A selective antiglioma activity of DVL is suggested by inhibiting U87 glioma cell migration and proliferation and inducing cell death, partially associated with autophagy, and likely involving Akt and mTORC1 dephosphorylation. Expand
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