Transient responses of hybridoma cells to nutrient additions in continuous culture: I. Glucose pulse and step changes.

@article{Miller1989TransientRO,
  title={Transient responses of hybridoma cells to nutrient additions in continuous culture: I. Glucose pulse and step changes.},
  author={William Marcus Miller and Charles R. Wilke and Harvey W. Blanch},
  journal={Biotechnology and bioengineering},
  year={1989},
  volume={33 4},
  pages={477-86}
}
Glucose and glutamine are the main nutrients used by mammalian cells in culture. Each provides unique biosynthetic precursors but are complementary for production of other metabolites and energy. The transient and steady-state responses of hybridoma growth and metabolism to glucose pulse and step changes have been examined. Metabolic quotients are reported for oxygen, glucose, lactate, ammonia, glutamine, alanine, and other amino acids. The glucose consumption rate increased by 100-200… CONTINUE READING
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