Transient responses of hybridoma cells in continuous culture to step changes in amino acid and vitamin concentrations

@article{Hiller1994TransientRO,
  title={Transient responses of hybridoma cells in continuous culture to step changes in amino acid and vitamin concentrations},
  author={G. Hiller and D. Clark and H. Blanch},
  journal={Biotechnology and Bioengineering},
  year={1994},
  volume={44}
}
The effects of step‐change increase in the concentrations of amino acids and vitamins on the metabolism, growth, and antibody productivity of a murine hybridoma cell line grown in continuous culture on serum‐free medium are presented. Additions of the amino acids cysteine with methionine, tryptophan, and isoleucine with valine and vitamin B12 (as cyanocobalamin) resulted in significant increases in viable cell concentrations. Additions of aspartate with asparagine, and threonine with vitamin B1… Expand
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