Transient receptor potential M3 channels are ionotropic steroid receptors in pancreatic β cells

  title={Transient receptor potential M3 channels are ionotropic steroid receptors in pancreatic $\beta$ cells},
  author={Thomas F.J. Wagner and Sabine Loch and Sachar Lambert and Isabelle Straub and Stefanie Mannebach and Ilka Mathar and Martina D{\"u}fer and Annette Lis and Veit Flockerzi and Stephan Ernst Philipp and Johannes Oberwinkler},
  journal={Nature Cell Biology},
Transient receptor potential (TRP) cation channels are renowned for their ability to sense diverse chemical stimuli. Still, for many members of this large and heterogeneous protein family it is unclear how their activity is regulated and whether they are influenced by endogenous substances. On the other hand, steroidal compounds are increasingly recognized to have rapid effects on membrane surface receptors that often have not been identified at the molecular level. We show here that TRPM3, a… 

Steroids and TRP Channels: A Close Relationship

Transient receptor potential channels are remarkable transmembrane protein complexes that are essential for the physiology of the tissues in which they are expressed and are subject to being affected by several endogenously-produced compounds.

Signal transduction via TRPM3 channels in pancreatic β-cells.

The view that TRPM3 represents an ionotropic steroid receptor of pancreatic β-cells linking insulin release with steroid hormone signaling is discussed and a direct effect of TR PM3 stimulation on insulin secretion has been reported.

Transient Receptor Potential Melastatin 1 (TRPM1) Is an Ion-conducting Plasma Membrane Channel Inhibited by Zinc Ions*

It is shown that heterologous expression of TRPM1 proteins induces ionic conductances that can be activated by extracellular steroid application, and it is demonstrated that TR PM1 proteins are bona fide ion-conducting plasma membrane channels.

Transient receptor potential cation channels in pancreatic β cells.

This review will focus on cation channels from the melastatin TRP -subfamily and discuss how TRPM channels can influence Ca(2+) signaling in β cells, and critically discuss the use of mouse models for the unraveling of basic mechanisms of insulin release.

Molecular Regulations and Functions of the Transient Receptor Potential Channels of the Islets of Langerhans and Insulinoma Cells

This review briefly compiles the current knowledge about the molecular mechanisms of regulations, and functions of the TRP channels in the β-cells, the α- cells, and some insulinoma cell lines.

Carboxamido steroids inhibit the opening properties of transient receptor potential ion channels by lipid raft modulation[S]

Transient Receptor Potential (TRP) cation channels, like the TRP Vanilloid 1 (TRPV1) and TRP Ankyrin 1 (TRPA1), are expressed on primary sensory neurons. These thermosensor channels play a role in

Regulation of TRP channels by steroids: Implications in physiology and diseases.

The Role of TRP Proteins in Mast Cells

Current knowledge about the expression of individual Trp genes with the majority of the 28 members being yet identified in different mast cell models are summarized, and mechanisms how they can regulate mast cell functions are highlighted.



TRP4 (CCE1) Protein Is Part of Native Calcium Release-activated Ca2+-like Channels in Adrenal Cells*

It is demonstrated that TRP4 contributes essentially to the formation of native CRAC-like channels in adrenal cells.

The TRP Superfamily of Cation Channels

Members of the TRP superfamily function in various processes, although their roles are best established in sensory modalities ranging from vision to hearing, taste, pheromone detection, pain perception, and osmosensation, which have relevance for human health.

Anandamide and arachidonic acid use epoxyeicosatrienoic acids to activate TRPV4 channels

It is shown that the endocannabinoid anandamide and its metabolite arachidonic acid activate TRPV4 in an indirect way involving the cytochrome P450 epoxygenase-dependent formation of epoxyeicosatrienoic acids.

Effects on membrane capacitance of steroids with antagonist properties at GABAA receptors.

It is suggested that negatively charged sulfated steroids alter the plasma membrane capacitance without physical movement of the molecule through the electric field.

Regulation of Insulin Secretion in Islets of Langerhans by Ca2+Channels

  • D. Mears
  • Biology
    The Journal of Membrane Biology
  • 2004
The emerging picture is that Ca2+ channels regulate insulin secretion at multiple sites in the stimulus-secretion coupling pathway, with the specific role of each channel determined by its biophysical and structural properties.

The Recombinant Human TRPV 6 Channel Functions as Ca 2 Sensor in Human Embryonic Kidney and Rat Basophilic Leukemia Cells *

TRPV6, expressed in human embryonic kidney cells and in rat basophilic leukemia cells, functions as a Ca sensing Ca channel independently of procedures known to deplete Ca stores.

Molecular and Functional Characterization of the Melastatin-related Cation Channel TRPM3*

From its function and expression in human kidney, a role of TRPM3 is proposed in renal Ca2+ homeostasis, suggesting volume-regulated activity of TRP3.

Activation of the Melastatin-Related Cation Channel TRPM3 by d-erythro-Sphingosine

Using measurements with the Ca2+-sensitive fluorescent dye fura-2 and the whole-cell patch-clamp technique, it is found that d-erythro-sphingosine, a metabolite arising during the de novo synthesis of cellular sphingolipids, activated TRPM3, the first ion channel activated by sphingoipids.

Alternative Splicing Switches the Divalent Cation Selectivity of TRPM3 Channels*

These results show that alternative splicing can be a mechanism to produce channels with very different selectivity profiles, and unambiguously show that TRPM3 proteins constitute a pore-forming channel subunit and localize the position of the ion-conducting pore within theTRPM3 protein.

Expression and Characterization of Human Transient Receptor Potential Melastatin 3 (hTRPM3)*

Results are consistent with the hypothesis that hTRPM3 mediates a Ca2- entry pathway that apparently is distinct from the endogenous Ca2+ entry pathways present in HEK 293 cells.