Transient gene expression in suspension HEK-293 cells: application to large-scale protein production.

@article{Baldi2005TransientGE,
  title={Transient gene expression in suspension HEK-293 cells: application to large-scale protein production.},
  author={L. Baldi and Natalie Muller and Sylviane Picasso and Raymond Jacquet and Philippe Girard and Huy Phan Thanh and Elisabeth Derow and F. M. Wurm},
  journal={Biotechnology progress},
  year={2005},
  volume={21 1},
  pages={148-53}
}
Recent advances in genomics, proteomics, and structural biology raised the general need for significant amounts of pure recombinant protein (r-protein). Because of the difficulty in obtaining in some cases proper protein folding in bacteria, several methods have been established to obtain large amounts of r-proteins by transgene expression in mammalian cells. We have developed three nonviral DNA transfer protocols for suspension-adapted HEK-293 and CHO cells: (1) a calcium phosphate based… CONTINUE READING

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