Transient gene expression in electroporated Picea glauca protoplasts

  title={Transient gene expression in electroporated Picea glauca protoplasts},
  author={Faouzi Bekkaoui and Marinus Pilon and {\'E}ric Lain{\'e} and Dr. P. Suvarna Raju and William L. Crosby and David I. Dunstan},
  journal={Plant Cell Reports},
The reporter gene for chloramphenicol acetyltransferase (CAT) was introduced into white spruce (Picea glauca (Moench) Voss.) protoplasts by electroporation. CAT transient gene expression was increased by increasing the concentration of pCaMVCN plasmid and was affected by the level of the applied voltage. Highest CAT activities were obtained after electroporation with a pulse of−1 having an exponential decay constant of approximately 105ms. Linearized plasmid constructs gave much higher… CONTINUE READING