Transient gene expression control: effects of transfected DNA stability and trans-activation by viral early proteins.

@article{Alwine1985TransientGE,
  title={Transient gene expression control: effects of transfected DNA stability and trans-activation by viral early proteins.},
  author={James C. Alwine},
  journal={Molecular and cellular biology},
  year={1985},
  volume={5 5},
  pages={1034-42}
}
The effects of trans-acting factors and transfected DNA stability on promoter activity were examined with chloramphenicol acetyl transferase (CAT) transient expression analysis. With cotransfection into CV-1P and HeLa cells, simian virus 40 T antigen, adenovirus E1a, and herpes-virus IE proteins were compared for their ability to trans-activate a variety of eucaryotic promoters constructed into CAT plasmids. T antigen and the IE protein were promiscuous activators of all the promoters tested… CONTINUE READING
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