Transient expression of the uidA gene in Pinus pinea cotyledons: A study of heterologous promoter sequences

Abstract

Transfer and expression of the β-glucuronidase gene (uidA) in cultured cotyledons of stone pine (Pinus pinea L.) was obtained by microprojectile bombardment. Conditions for optimum transient expression were established by using plasmid pBI121 delivered by 1.0 μm-diameter gold particles, into 1-day-old cultured cotyledons. Helium pressure of 6.2 MPa… (More)
DOI: 10.1023/A:1006250609262

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@article{Humara2004TransientEO, title={Transient expression of the uidA gene in Pinus pinea cotyledons: A study of heterologous promoter sequences}, author={Jaime M. Humara and Mari{\'a}n L{\'o}pez and Ricardo Javier Ord{\'a}s}, journal={Plant Cell, Tissue and Organ Culture}, year={2004}, volume={56}, pages={69-78} }