Transient expression of lacZ in particle bombarded Gracilaria changii (Gracilariales, Rhodophyta)

Abstract

Using a Biolistic PDS 1000/He system, healthy thalli of Gracilaria changii were bombarded with gold particles coated with plasmid DNA containing the lacZ reporter gene. Transient expression of lacZ was observed in bombarded thalli under the rupture-disc pressures of 4482, 6206, 7584 and 8963 KPa, two days after bombardment. Although G. changii exhibits a slight blue background, positive expression and the background colour can be clearly differentiated. The results indicate that lacZ could be a useful reporter gene and that SV40 promoter could be an effective promoter for Gracilaria transformation.

DOI: 10.1023/A:1025141913156

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Cite this paper

@article{Gan2004TransientEO, title={Transient expression of lacZ in particle bombarded Gracilaria changii (Gracilariales, Rhodophyta)}, author={Sook-Yee Gan and Song Qin and Rofina Yasmin Othman and Daozhan Yu and Siew-Moi Phang}, journal={Journal of Applied Phycology}, year={2004}, volume={15}, pages={345-349} }