Transient and stable transfection of Leishmania by particle bombardment.

@article{Sbicego1998TransientAS,
  title={Transient and stable transfection of Leishmania by particle bombardment.},
  author={Sandro Sbicego and Achim Schnaufer and Beat Blum},
  journal={Molecular and biochemical parasitology},
  year={1998},
  volume={94 1},
  pages={
          123-6
        }
}
Transfection of trypanosomatids, particularly the transfection of their mitochondrion and different developmental stages, is still a limiting step in many experiments using recombinant DNA technology. Two types of trypanosomatid transfection protocols have been described so far. The standard protocol is based on electroporation first described by Bellofatto and Cross [1]. The second protocol is based on transfection using a homemade particle gun [2]. The biolistic method was first applied to… CONTINUE READING
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