Transient and Stable Expression of the Firefly Luciferase Gene in Plant Cells and Transgenic Plants

  title={Transient and Stable Expression of the Firefly Luciferase Gene in Plant Cells and Transgenic Plants},
  author={David W. Ow and Jeffrey R. de Wet and Donald R. Helinski and Stephen H. Howell and Keith V. Wood and Marlene Deluca},
  pages={856 - 859}
The luciferase gene from the firefly, Photinus pyralis, was used as a reporter of gene expression by light production in transfected plant cells and transgenic plants. A complementary DNA clone of the firefly luciferase gene under the control of a plant virus promoter (cauliflower mosaic virus 35S RNA promoter) was introduced into plant protoplast cells (Daucus carota) by electroporation and into plants (Nicotiana tabacum) by use of the Agrobacterium tumefaciens tumor-inducing plasmid. Extracts… 
Transient expression assay in a baculovirus system using firefly luciferase gene as a reporter
It is found that flanking sequences of the AcNPV DNA in the transfer plasmid contained an unknown promoter conferring an efficient luc expression, which allowed for highly sensitive monitoring of the transient expression of foreign genes from the transfecting plasmids prior to construction of recombinant viruses.
Isolation and expression in transgenic tobacco and rice plants, of the cassava vein mosaic virus (CVMV) promoter
The expression pattern of the CVMV promoters was analyzed in transgenic tobacco and rice plants, and revealed that the GUS staining pattern was similar for each construct and in both plants, suggesting a near constitutive pattern of expression.
Molecular Biology Expression of Two Tissue-Specific Promoters in Transgenic Cotton Plants
Results indicate that these two tissue-specific promoters have the potential to be used to differentially express interesting genes in developing seeds or leaves of transgenic cotton.
Enhanced stable expression luciferase under the control fMranslational enhancer in plants of a Vibrio of the transgenic
The results show that a specific protein from an introduced foreign gene can be produced with high efficiency in cultivated plants and such a system is therefore amenable for production of desired proteins through conventional farming methods.
Construction and testing of an intron-containing luciferase reporter gene fromRenilla reniformis
Results demonstrate that theiLUC reporter gene can be used to monitor luciferase expression in transient and stable transformation experiments without interference from contaminating agrobacteria.
The use of the luciferase reporter system forin planta gene expression studies
It is shown when in planta luciferase activity is measured under substrate equilibrium conditions and can be related to the promoter activity of the reporter gene, which gives the prerequisites for the correct utilisation of the luciferases reporter system.
The laccase promoter of potato confers strong tuber-specific expression in transgenic plants
The data show that the laccase promoter represents a feasible candidate to drive high and preferential expression of genes in potato tubers and indicates that the tuber-specific regulatory elements might be scattered throughout the promoter.
Luciferase Gene Expressed in Plants, Not in Agrobacterium
The luciferase gene from the North American firefly Photinus pyralis has been used as a reporter gene in a variety of eukaryotic and prokaryotic organisms and provides a more dynamic indication of in vivo mRNA levels than the longer lived β-glucuronidase (GUS) or green fluorescent protein (GFP) reporter proteins.


Cloning of firefly luciferase cDNA and the expression of active luciferase in Escherichia coli.
A cDNA library was constructed from firefly (Photinus pyralis) lantern poly(A)+ RNA, using the Escherichia coli expression vector lambda gt11, and sufficient coding information was contained to direct the synthesis of active firefly luciferase in E. coli.
Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene.
The results of these experiments indicate that plants can be genetically transformed for resistance to virus disease development.
Light-regulated and organ-specific expression of a wheat Cab gene in transgenic tobacco
The light-regulated and organ-specific expression of a monocotyledonous gene in transgenic dicotylingonous plants is reported for the first time.
Developmentally regulated expression of the bean beta-phaseolin gene in tobacco seed.
The phaseolin gene appeared to be inserted as a single copy, and the proportion of phaseolin per genome copy in tobacco seeds resembled that in the bean seeds, which is 40% of total seed protein, expressed from about 14 copies per diploid genome.
Expression in plants of a mutant aroA gene from Salmonella typhimurium confers tolerance to glyphosate
An example of how a gene for herbicide tolerance, an agronomically important trait, can be found in a bacterium and introduced into plants is presented.
Measuring gene expression with light.
Genes in Escherichia coli and Vibrio parahaemolyticus were mutagenized, and mutants containing transposon-generated lux gene fusions produced light as a function of target gene transcription.
Organ-Specific and Light-Induced Expression of Plant Genes
An enhancer-like element contained within a 240—base pair fragment can confer phytochrome-induced transcription and organ specificity on nonregulated promoters in plants.
Developmental regulation of two genes encoding ribulose-bisphosphate carboxylase small subunit in pea and transgenic petunia plants: Phytochrome response and blue-light induction.
  • R. FluhrN. Chua
  • Environmental Science
    Proceedings of the National Academy of Sciences of the United States of America
  • 1986
The two pea rbcS genes were transferred into the petunia genome and it was found that their phytochrome and blue-light responses are recapitulated in the appropriate developmental stages of the transgenic plants.
Binary Agrobacterium vectors for plant transformation.
  • M. Bevan
  • Biology
    Nucleic acids research
  • 1984
A vector molecule for the efficient transformation of higher plants has been constructed with several features that make it efficient to use. It utilizes the trans acting functions of the vir region
Photosynthesis-Associated Gene Families: Differences in Response to Tissue-Specific and Environmental Factors
The results show that plastid development plays a crucial role in the activation of expression of these chimeric genes in transgenic tobacco plants, and particular members of each of the above gene families respond differently to tissue-specific and environmental factors.