Transient and Stable Expression of the Firefly Luciferase Gene in Plant Cells and Transgenic Plants

@article{Ow1986TransientAS,
  title={Transient and Stable Expression of the Firefly Luciferase Gene in Plant Cells and Transgenic Plants},
  author={David W. Ow and Jeffrey R. de Wet and Donald R. Helinski and Stephen H. Howell and Keith V. Wood and Marlene Deluca},
  journal={Science},
  year={1986},
  volume={234},
  pages={856 - 859}
}
The luciferase gene from the firefly, Photinus pyralis, was used as a reporter of gene expression by light production in transfected plant cells and transgenic plants. A complementary DNA clone of the firefly luciferase gene under the control of a plant virus promoter (cauliflower mosaic virus 35S RNA promoter) was introduced into plant protoplast cells (Daucus carota) by electroporation and into plants (Nicotiana tabacum) by use of the Agrobacterium tumefaciens tumor-inducing plasmid. Extracts… 
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