Transient alterations in cellular permeability in cultured human proximal tubule cells: Implications for transport studies

@article{Morshed1995TransientAI,
  title={Transient alterations in cellular permeability in cultured human proximal tubule cells: Implications for transport studies},
  author={Khandoker M. Morshed and Kenneth E. McMartin},
  journal={In Vitro Cellular & Developmental Biology - Animal},
  year={1995},
  volume={31},
  pages={107-114}
}
Primary cultures of human proximal tubule (HPT) cells possess the characteristics of a tight epithelium and retain the characteristics of in vivo renal function. HPT cells from confluent monolayers when grown on collagen-coated polycarbonate inserts in a hormonally defined serum-free medium. However, initial studies of transepithelial transport observed large bidirectional fluxes of the paracellular probe inulin. The present studies were designed to assess the transformation of HPT cell tight… CONTINUE READING