Transgenic zebrafish produced by retroviral infection of in vitro-cultured sperm.

@article{Kurita2004TransgenicZP,
  title={Transgenic zebrafish produced by retroviral infection of in vitro-cultured sperm.},
  author={Kayoko Kurita and Shawn M. Burgess and Noriyoshi Sakai},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2004},
  volume={101 5},
  pages={1263-7}
}
Transgenic modification of sperm before fertilization has distinct advantages over conventional transgenic methods. The primary advantage is that the mosaicism inherent in those other techniques is avoided. A culture system using primary cultures of zebrafish male germ cells, in which the differentiation from spermatogonia to functional sperm can occur in vitro, provides the opportunity for genetic modification of sperm in vitro. Here, we report the production of transgenic zebrafish from… CONTINUE READING
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