Transgenic tobacco plants expressing the Arabidopsis thaliana nitrilase II enzyme.

@article{Schmidt1996TransgenicTP,
  title={Transgenic tobacco plants expressing the Arabidopsis thaliana nitrilase II enzyme.},
  author={Ronny Schmidt and Axel M{\"u}ller and Ruediger Hain and Dieter Bartling and Elmar W. Weiler},
  journal={The Plant journal : for cell and molecular biology},
  year={1996},
  volume={9 5},
  pages={683-91}
}
Nitrilase (E.C. 3.5.5.1) cloned from Arabidopsis thaliana converts indole-3-acetonitrile to the plant growth hormone, indole-3-acetic acid in vitro. To probe the capacity of ths enzyme under physiological conditions in vivo, the cDNA PM255, encoding nitrilase II, was stably integrated into the genome of Nicotiana tabacum by direct protoplast transformation under the control of the CaMV-35S promotor. The regenerated plants appeared phenotypically normal. Nitrilase II was expressed, based on the… CONTINUE READING