Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system

  title={Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system},
  author={J. Livet and Tamily A. Weissman and Hyuno Kang and Ryan W Draft and Ju Lu and Robyn A. Bennis and Joshua R Sanes and Jeff William Lichtman},
Detailed analysis of neuronal network architecture requires the development of new methods. Here we present strategies to visualize synaptic circuits by genetically labelling neurons with multiple, distinct colours. In Brainbow transgenes, Cre/lox recombination is used to create a stochastic choice of expression between three or more fluorescent proteins (XFPs). Integration of tandem Brainbow copies in transgenic mice yielded combinatorial XFP expression, and thus many colours, thereby… CONTINUE READING
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